eBERlight is a U.S. Department of Energy (DOE)’s Office of Science Biologicaland Environmental Research (BER) resource operating at the AdvancedPhoton Source. The program provides access and user support for severalx-ray-based tools for research in biological, geological, geochemical, andenvironmental sciences, such as:

  • Macromolecular Crystallography (MX)
  • X-ray Microscopy (X-fluorescence microscopy, XFM, and ptychography)
  • X-ray Full-Field Imaging (Computed Tomography, CT)
  • X-ray scattering (Small/Wide-Angle X-ray Scattering, SAXS/WAXS)
  • X-ray absorption spectroscopy (XAS)

Leveraging additional resources available at Argonne National Laboratoryfor sample preparation and data analysis, eBERlight creates acomprehensive ecosystem to meet the needs of complex environmentaland biological research projects, and to take full advantage of the upgradedlight source.

APS eBERlight fact sheet


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Results in this report are derived from work performed through the eBERlight program, funded by the U.S. Department of Energy, Office of Biological and Environmental Research at the Advanced Photon Source, a U.S. DOE Office of Science User Facility, supported by the U.S. DOE, Office of Basic Energy Sciences, under Contract No. DE-AC02-06CH11357.


eBERlight is accepting proposals through the Advanced Photon Source (APS) Universal Proposal System (UPS). Proposal calls will be open this Spring, to researchers with a focus on areas aligned to the DOE-BER mission. This includes biological, geological, geochemical and environmental sciences, to address existing and new scientific challenges.

Researchers at eBERlight are working with the Facilities Integrating Collaborations for User Science (FICUS) program to provide researchers with access to world-class resources at Environmental Molecular Sciences Laboratory (EMSL), Joint Genome Institute (JGI), the National Ecological Observatory Network (NEON), the Bio-SANS beamline at the High Flux Isotope Reactor (HFIR) through the Center for Structural Molecular Biology (CSMB), and the Advanced Photon Source (APS).FICUS proposal calls are announced annually. The FY25 call is open for users. Visit the Proposal Calls page for more information.

The2024 APS/CNM Users Meeting will be held, May 6-10, 2024.  Register before April 22.  Hosted workshops,

APS WK#9: Using Synchrotron Light to Probe Earth and Environmental Systems with Dr. Zou Finfrock.

APS WK#11: APS-U-enabled Advanced Tools for Structural Biology: Advancing Enzymatic Catalysis and Drug Discovery through Synchrotron Serial Crystallography with Dr. Karolina Michalska.

Training and Outreach

Scientists working with eBERlight play a pivotal role in pioneering innovative scientific methodologies. eBERlight recognizes that talent is increasingly diverse. Taking benefit of  Argonne’s educational programs, the laboratory foster’s a welcoming and inclusive environment that offers a variety of educational and outreach programs to cultivate the next generation of scientists and engineers.  These programs introduce students, early-career scientists, and engineers to mission-driven, high-impact research. There are a variety of research opportunities to work alongside scientific mentors at the laboratory in a paid internship appointment.  Students and graduates are encouraged to apply online, or contact eBERlightfor opportunities available.

Graduate students currently pursuing Ph.D. degrees in areas of physics, chemistry, material sciences, biology (non-medical), mathematics, engineering, computer or computational sciences, or specific areas of environmental sciences that are aligned with the mission of the Office of Science are eligible to apply for the supplemental research awards provided by the Office of Science Graduate Student Research (SCGSR) program.   The next solicitation opens in May 2024.

Supported Techniques
Macromolecular Crystallography MX / SSX
X-ray Microscopy X-fluorescence microscopy, XFM, and ptychography
X-ray Full-Field Imaging Computed Tomography, CT
X-ray scattering Small/Wide-Angle X-ray Scattering, SAXS/WAXS
X-ray absorption spectroscopy XAS
Science Highlights

Structure and enzymatic characterization of CelD endoglucanase from the anaerobic fungus Piromyces finnis
Dementiev A, Lillington SP, Jin S, Kim Y, Jedrzejczak R,Michalska K, Joachimiak A, O'Malley MA.Appl Microbiol Biotechnol. 2023 Oct;107(19):5999-6011. doi: 10.1007/s00253-023-12684-0. PMID: 37548665

A high-throughput structural system biology approach to increase structure representation of proteins from Clostridioides difficile
Monica Rosas-Lemus, Supratim Dey, George Minasov, Kemin Tan, Spencer M. Anderson, Joseph Brunzelle, Salvatore Nocadello, Ivan Shabalin, Ekaterina Filippova, Andrei Halavaty, Youngchang Kim, Natalia Maltseva, Jerzy Osipiuk, Wladek Minor, Andrzej Joachimiak, Alexei Savchenko, Wayne F. Anderson, Karla J.F. Satchell, J. Bacteriol. 12 (10), e00507 (2023). DOI: 10.1128/MRA.00507-23

Partnering Beamline Publications

Nicole L. Inniss, Travis J. Kochan, George Minasov, Zdzislaw Wawrzak, Changsoo Chang, Kemin Tan, Ludmilla Shuvalova, Olga Kiryukhina, Sergii Pshenychnyi, Ruiying Wu, Ivegeniia Dubrovska, Gyorgy Babnigg, Michael Endres, Wayne F. Anderson, Alan R. Hauser, Andrzej Joachimiak, Karla J.F. Satchell, "A Structural Systems Biology Approach to High-Risk CG23 Klebsiella pneumoniae," Microbiology 12 (2), e01013 (2023). DOI: 10.1128/mra.01013-22

Doga Yamac Ozgulbas, Don Jensen Jr., Rory Butler, Rafael Vescovi, Ian T. Foster, Michael Irvin, Yasukazu Nakaye, Miaoqi Chu, Eric M. Dufresne, Soenke Seifert, Gyorgy Babnigg, Arvind Ramanathan, Qingteng Zhang, "Robotic pendant drop: containerless liquid for [mu]s-resolved, AI-executable XPCS," Light Sci. Appl. 12, 196 (2023). DOI: 10.1038/s41377-023-01233-z

Vanessa Cupil-Garcia, Joy Q. Li, Stephen J. Norton, Ren A. Odion, Pietro Strobbia, Luca Menozzi, Chenshuo Ma, Jianhong Hu, Rodolfo Zentella, Maxim I. Boyanov, Y. Zou Finfrock, Doga Gursoy, Deirdre Sholto Douglas, Junjie Yao, Tai-Ping Sun, Kenneth M. Kemner, Tuan Vo-Dinh, "Plasmonic nanorod probes’ journey inside plant cells for in vivo SERS sensing and multimodal imaging," Nanoscale <15 (13), 6396-6407 (2023). DOI: 10.1039/D2NR06235F

Pawel M. Wydorski, Jerzy Osipiuk, Benjamin T. Lanham, Christine Tesar, Michael Endres, Elizabeth Engle, Robert Jedrzejczak, Vishruth Mullapudi, Karolina Michalska, Krzysztof Fidelis, David Fushman, Andrzej Joachimiak, Lukasz A. Joachimiak, "Dual domain recognition determines SARS-CoV-2 PLpro selectivity for human ISG15 and K48-linked di-ubiquitin," Nat. Commun. <14, 2366 (2023). DOI: 10.1038/s41467-023-38031-5

B. Ellyson, A. Saville, K. Fezzaa, T. Sun, N. Parab, C. Finfrock, C.J. Rietema, D. Smith, J. Copley, C. Johnson, C.G. Becker, J. Klemm-Toole, C. Kirk, N. Kedir, J. Gao, W. Chen, K.D. Clarke, A.J. Clarke, "High strain rate deformation of aged TRIP Ti-10V-2Fe-3Al (wt.%) examined by in-situ synchrotron X-ray diffraction," Acta Mater. <245, 118621 (2023). DOI: 10.1016/j.actamat.2022.118621

Priya Karna, Zou Finfrock, Junjie Li, Yongfeng Hu, Dong-Sheng Yang, "Water-Soluble Copper(I) Hydroxide Catalyst and Its Formation in Ligand-Free Suzuki–Miyaura Cross-Coupling Reactions," J. Phys. Chem. C <127 (12), 5791-5799 (2023). DOI: 10.1021/acs.jpcc.3c00268

Adnan Ozden, Jun Li, Sharath Kandambeth, Xiao-Yan Li, Shijie Liu, Osama Shekhah, Pengfei Ou, Y. Zou Finfrock, Ya-Kun Wang, Tartela Alkayyali, F. Pelayo García de Arquer, Vinayak S. Kale, Prashant M. Bhatt, Alexander H. Ip, Mohamed Eddaoudi, Edward H. Sargent, David Sinton, "Energy- and carbon-efficient CO[subscript 2]/CO electrolysis to multicarbon products via asymmetric ion migration–adsorption," Nat. Energy <8, 179-190 (2023). DOI: 10.1038/s41560-022-01188-2 

Peter J Stogios, Sean D Liston, Cameron Semper, Bradley Quade, Karolina Michalska, Elena Evdokimova, Shane Ram, Zbyszek Otwinowski, Dominika Borek, Leah E Cowen, Alexei Savchenko, "Molecular analysis and essentiality of Aro1 shikimate biosynthesis multi-enzyme in Candida albicans," Life Sci. Alliance <5 (8), e202101358 (2022). DOI: 10.26508/lsa.202101358

Nicholas L. Bartelli, Victor J. Passanisi, Karolina Michalska, Kiho Song, Dinh Q. Nhan, Hongjun Zhou, Bonnie J. Cuthbert, Lucy M. Stols, William H. Eschenfeldt, Nicholas G. Wilson, Jesse S. Basra, Ricardo Cortes, Zainab Noorsher, Youssef Gabraiel, Isaac Poonen-Honig, Elizabeth C. Seacord, Celia W. Goulding, David A. Low, Andrzej Joachimiak, Frederick W. Dahlquist, Christopher S. Hayes, "Proteolytic processing induces a conformational switch required for antibacterial toxin delivery," Nat. Commun. <13, 5078 (2022). DOI: 10.1038/s41467-022-32795-y 

Ariana D. Forand, Y. Zou Finfrock, Miranda Lavier, Jarvis Stobbs, Li Qin, Sheng Wang, Chithra Karunakaran, Yangdou Wei, Supratim Ghosh, Karen K. Tanino, "With a Little Help from My Cell Wall: Structural Modifications in Pectin May Play a Role to Overcome Both Dehydration Stress and Fungal Pathogens," Plant J. 11 (3), 385 (2022). DOI: 10.3390/plants11030385 


The APS Publications Database  is the official source for listing APS-related journal articles, conference proceedings and papers, dissertations, books, book chapters, technical reports, abstracts, awards, invited talks, patents, magazine articles, and newsletters in order to produce a comprehensive record about research at the APS.